Maize Dwarf Mosaic Disease Occurred in Hangzhou Isolate Caused by Sugarcane M osaic Virus

Recently maize dwarf mosaic disease WaS occurred on maize crop seriously in large scale in Hangzhou district．Purified preparations from the infected maize leaves~ontained numerous filamen— t0us virus particles of e 750 nm in leng th．Cells of infected plants contained typical pinwheels and lam— inated aggregates．The coat protein of the virus was 33．6 kD A 1．8 kb fragment of 3’一terminus of the viral RNA was amplified by RT—PCR，cloned and its sequence was determined。Sequence compar— isons showed that it shared 71 5％ -99 1％ homology with isolates of sugarcane mosaic virus．67。8％ ～ 68．5％ with sorghum mosaic virus and 38。4％ ～48．4％ with ma ize dwarf mosaic virus，indicating that the pathogen of this disease on maize in Hangzhou was sugarcane mosaic virus．In addition．the relationships of sugarcane mosaic virus isolates from different origins all OVer world were discussed based 0n coat protein sequences