Studies on Apoptosis of Host Cells Induced by Foot-and-M outh Disease Virus

Apoptosis of PK—t5 ceils induced by Foot—and-Mouth Disease Virus(FMDV)in vitro was reported in this paper．Typical cell apoptosis was detected hy ti．se of Hoechst 33258 fluorescence probe． agarose gel electrophoresis and in situ end-labeling(TUNEL)．After PK一15 cells were infected by titration of 4．8 lg TCIDsD／mL FMDV for 32 h，apoptosis characteristics of nuclear condensation， fragmentation，accompanied by apoptotic bodies formation(Hoeehst 33258 staining)．180—200 inte— ger-fold sized pieces DNA Ladders(agarose gel e1．ectrophoresis)and strong green fluorescence clots (TUNEL)were alI exhibited，and cell apoptosis was approximately 20％ In addition．the quantira— tire analysis of apoptosis in PK一15 ceils induced by FMDV showed that apoptosis was correlated with infection of virus．and it was also tim~dependent．Results indicare that FMDV can induce apoptosis of host ceils and apoptosis plays an important role in the cytopathogencity effect of FMDV