Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood
Abstract: Two pairs of primers were used for amplifying SFV gene with nested—PCR in Rhesus Mon— key blood The neady 500 bp PCR products were cloned into vector pUC-19.The fragment was de tected as 465 bp SFV pol gene by sequence analysis.The homology of the fragment to 425 bp pol gene of the SFV-1 was the highest.it was 92 00% 158 samples of Rhesus Monkey blood were used to de teet SFV gene with nested—PCR Among them,54 were positive.the poskive rate was 34 2% h ap— peared a h/gh present of SFV infection in Rhesus M onkey.