Citation: REN Li—hong.BAI W ei, LIN Ling, SUN Ming, LI Hua, YI Hong—kun, HOU Zhong—liu, . Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood .VIROLOGICA SINICA, 2001, 16(2) : 179-182.

Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood

  • Available online: 05 June 2001
  • Two pairs of primers were used for amplifying SFV gene with nested—PCR in Rhesus Mon— key blood The neady 500 bp PCR products were cloned into vector pUC-19.The fragment was de tected as 465 bp SFV pol gene by sequence analysis.The homology of the fragment to 425 bp pol gene of the SFV-1 was the highest.it was 92 00% 158 samples of Rhesus Monkey blood were used to de teet SFV gene with nested—PCR Among them,54 were positive.the poskive rate was 34 2% h ap— peared a h/gh present of SFV infection in Rhesus M onkey.

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    Detection of SFV Gene with Nested·PCR in Rhesus M onkey Blood

    • 1. Institute of Medical Biology,CAMS & PUMC.Kunming 650118.China

    Abstract: Two pairs of primers were used for amplifying SFV gene with nested—PCR in Rhesus Mon— key blood The neady 500 bp PCR products were cloned into vector pUC-19.The fragment was de tected as 465 bp SFV pol gene by sequence analysis.The homology of the fragment to 425 bp pol gene of the SFV-1 was the highest.it was 92 00% 158 samples of Rhesus Monkey blood were used to de teet SFV gene with nested—PCR Among them,54 were positive.the poskive rate was 34 2% h ap— peared a h/gh present of SFV infection in Rhesus M onkey.

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