Citation: HU Guo-dong, WANG Li—hua, HU Xiao-hui, YANG Kaia, PANG Yi. Nucleotide Sequence of Spodoptera litura Nucleopolyhedrovirus DNA Xba I 4.0 kb Containing the Zinc Finger Protein Gene and Repetitive Sequence Regions .VIROLOGICA SINICA, 2001, 16(3) : 236-241.

Nucleotide Sequence of Spodoptera litura Nucleopolyhedrovirus DNA Xba I 4.0 kb Containing the Zinc Finger Protein Gene and Repetitive Sequence Regions

  • Available online: 05 September 2001
  • The complete nucleotide sequence of Spodoptera litura nueleopolyhedrovims(SpltNPV)DNA Xba I 4.0 kb has been determined.It contains a zinc finger protein gene and three repetitive sequence regions·The open madil1g frame of this zinc finger gene is 2 196 bp long,enc0d_mg a putative protein of 731 amino acids withmo lecular wei出0f 83.09 kD andisodeeⅡic point of4.61.The 5 noncoding region ofthis gene has olle baculovims early promotermotif(GAGT)and olle TATA box.Five polyadenylafion sitwats,AATAAA,arefound inthe do~ stream ofthetranslation stop codon.A ringfingermotifwhichis a C3HG4type of zincfinger pm— telnlnotifand alqUOlearlocalization si 丑l present atthe am/no acid residues 232~241 and 323~340 — spectively.This proteinis probablyhi y curled.Th repetitive SG~XleneA2:regions(SR1,SR2,SR3)are present in this fragment.SRI and SR3 contain nlllTl~rous repetitive SOqlfleDCe$,,and one of these in SRI is 41bp long. SR1 and SR3 possibly act且s the transcfiption aI enha兀ceI音or or;~s of replication.

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    Nucleotide Sequence of Spodoptera litura Nucleopolyhedrovirus DNA Xba I 4.0 kb Containing the Zinc Finger Protein Gene and Repetitive Sequence Regions

    • 1. State key laboratory for Biocontrol and lnstitute of Entomology,Zhongshan University,Guangzhou 510275,China

    Abstract: The complete nucleotide sequence of Spodoptera litura nueleopolyhedrovims(SpltNPV)DNA Xba I 4.0 kb has been determined.It contains a zinc finger protein gene and three repetitive sequence regions·The open madil1g frame of this zinc finger gene is 2 196 bp long,enc0d_mg a putative protein of 731 amino acids withmo lecular wei出0f 83.09 kD andisodeeⅡic point of4.61.The 5 noncoding region ofthis gene has olle baculovims early promotermotif(GAGT)and olle TATA box.Five polyadenylafion sitwats,AATAAA,arefound inthe do~ stream ofthetranslation stop codon.A ringfingermotifwhichis a C3HG4type of zincfinger pm— telnlnotifand alqUOlearlocalization si 丑l present atthe am/no acid residues 232~241 and 323~340 — spectively.This proteinis probablyhi y curled.Th repetitive SG~XleneA2:regions(SR1,SR2,SR3)are present in this fragment.SRI and SR3 contain nlllTl~rous repetitive SOqlfleDCe$,,and one of these in SRI is 41bp long. SR1 and SR3 possibly act且s the transcfiption aI enha兀ceI音or or;~s of replication.

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