Sequence Analysis of the Helicoverpa armigera Nuclear Polyhedrosis Virusp40 Gene
Abstract: The Hind Ⅲ-H?J fragments of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HaSNPV) qenome were cloned. By sequencing the terminus of the cloned fragments, an ORF encoding the HaSNPV p40 was identified. The coding region was 966 bp long and potential to encode a 36.6kD pepetide. The HaSNPV p40 exhibits 98% identity with HzSNPV p40 at the nucleotide sequence. The two genes diverged significantly from BmNPV p40 and AcMNPV gp41.The predicted amino acid sequences have less than 45% homology between HaSNPV p40?HzSNPV p40 and BmNPV p40 or AcMNPV gp41, but four genes have a conserved hydrophilic domain. In this region they have 62% homology. Further more, we determined that Hind Ⅲ-H fragment links with J fragment in the genome and the two fragments were analasysed with four restriction endonucleases (BamH Ⅰ?EcoR Ⅰ?Hind Ⅲ?Pst Ⅰ) .