Citation: ZHOU Peng-cheng, NIE Yu—chun, CAO Qi, CHEN Jian·guo, DING Ming—xiao. Study On the Inhibition of Specific 01igonucleotides On Classical Swine fever Virus in vitro .VIROLOGICA SINICA, 2001, 16(3) : 270-274.

Study On the Inhibition of Specific 01igonucleotides On Classical Swine fever Virus in vitro

  • Available online: 05 September 2001
  • The effect of oligodeoxynuclcotldes(ODN)OI1 replication of CSFV(Shimen strain)WSIS investigated in this papor.Ph~phorthimte 01igedeox.vnuel·eoddes(PS-ODN)targeted to the~gion of the 3’,5._nonceding re,on(NCR)as well SIS PS-ODNtargetedto NS3 gone region㈨maIke叭inhibitedthereplication of CSFV in PK-15 cells.Antiviral activity of PS·ODN Writs evaluated bY fluorescent plaque fomaation assav.The results presented here indicated that ODN 175 complementary to 5’temalnal conserved sequence mad it’s sense ODN P5S。as wel as1’8 complementartoNS3 region exhibited strongly antiviral activity,whereasthe anfiviral acfivi一 押ofP3 andit’s enese PS-ODN W86 muchlower.Atthe sametime,antMral activity of sense ODN is sli higher than corresponding antlsense ODN The inhibition ofODN is in a dose·and sequence—dependant]xlanller. Inhibition of CSFV’s replication in PK一15 ceils Call be signllicanfly increased in the presense of lipofecfin by either sense or anfisense 0DN.These data suggested both 5’·NCR and 3I_NCR may play important roles in CSFV replication.

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    Study On the Inhibition of Specific 01igonucleotides On Classical Swine fever Virus in vitro

    • 1. College of Life Science,Peking University,Beijing 100871,China

    Abstract: The effect of oligodeoxynuclcotldes(ODN)OI1 replication of CSFV(Shimen strain)WSIS investigated in this papor.Ph~phorthimte 01igedeox.vnuel·eoddes(PS-ODN)targeted to the~gion of the 3’,5._nonceding re,on(NCR)as well SIS PS-ODNtargetedto NS3 gone region㈨maIke叭inhibitedthereplication of CSFV in PK-15 cells.Antiviral activity of PS·ODN Writs evaluated bY fluorescent plaque fomaation assav.The results presented here indicated that ODN 175 complementary to 5’temalnal conserved sequence mad it’s sense ODN P5S。as wel as1’8 complementartoNS3 region exhibited strongly antiviral activity,whereasthe anfiviral acfivi一 押ofP3 andit’s enese PS-ODN W86 muchlower.Atthe sametime,antMral activity of sense ODN is sli higher than corresponding antlsense ODN The inhibition ofODN is in a dose·and sequence—dependant]xlanller. Inhibition of CSFV’s replication in PK一15 ceils Call be signllicanfly increased in the presense of lipofecfin by either sense or anfisense 0DN.These data suggested both 5’·NCR and 3I_NCR may play important roles in CSFV replication.

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