Citation: LI Ming, SHEN Xiao-chuan, LIN Jiao-jiao, NI Zhen-ya, YUAN Chun-xiu, LIU Jin-ming, YANG Guan-zhen, WU Xiang-fu, . Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression .VIROLOGICA SINICA, 2001, 16(3) : 283-285.

Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression

  • Available online: 05 September 2001
  • DNA fragments of Core(300bp)and NS4(400bp),encoding the nucleocapsid region(Core)protein CS and nonstructural region(NS4)protein NS4a of HCV,have been obtained from HCV genome by PCR,both of the two fragments were liked with C33c(700bp)and formed a chineric gene C33c-Core-NS4(HCV-CCN).The chimeric gene was recombined with expression vector pET24a(+)and was expressed in Escherichia coli.The expressed protein was purified by Ni(+)NTB affinity chromatography.Its molecular weight was about 58kD.Western blotting analysis showed that the chimerical antigen had good antigenicity,which could play an important role in anti-HCV assay.

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    Construction of a Chimeric Gene encoding Core,NS3 and NS4 Region Antigens of Hepatitis C Virus and Its Expression

    • 1. 1.上海动物生物技术研究中心,中国农业科学院上海家畜寄生虫病研究所,农业部动物寄生虫学重点开放实验室.上海 200232
    • 2. 中国科学院上海生化研究所.上海200031

    Abstract: DNA fragments of Core(300bp)and NS4(400bp),encoding the nucleocapsid region(Core)protein CS and nonstructural region(NS4)protein NS4a of HCV,have been obtained from HCV genome by PCR,both of the two fragments were liked with C33c(700bp)and formed a chineric gene C33c-Core-NS4(HCV-CCN).The chimeric gene was recombined with expression vector pET24a(+)and was expressed in Escherichia coli.The expressed protein was purified by Ni(+)NTB affinity chromatography.Its molecular weight was about 58kD.Western blotting analysis showed that the chimerical antigen had good antigenicity,which could play an important role in anti-HCV assay.

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