Citation: ZHAO Xiao-dong, ZHOU Jian-hua, DING Qing-quan. Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus .VIROLOGICA SINICA, 2001, 16(3) : 286-288.

Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

  • Available online: 05 September 2001
  • The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector.Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP,the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D,EcoR[-A,‰ dⅢ一A,Pst LD and Xho tG/H respectively.Atthe time,the fragment ofBamH I-D eontniningthe chiA has been cloned.

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    Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

    • 1. 1.Institute of virology,Chinese Academy of Sciences,Wuhan 430071,China 2.Sichuan Academy of sciences,Chendu 610081,China

    Abstract: The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector.Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP,the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D,EcoR[-A,‰ dⅢ一A,Pst LD and Xho tG/H respectively.Atthe time,the fragment ofBamH I-D eontniningthe chiA has been cloned.

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