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Volume 16 Issue 3
June 2001
    Citation: ZHAO Xiao-dong, ZHOU Jian-hua, DING Qing-quan. Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus .VIROLOGICA SINICA, 2001, 16(3) : 286-288.
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    Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

    • 1.

      1．Institute of virology，Chinese Academy of Sciences，Wuhan 430071，China 2．Sichuan Academy of sciences，Chendu 610081，China

    • Available online: 05 September 2001
    • The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector．Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP，the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D，EcoR[-A，‰ dⅢ一A，Pst LD and Xho tG／H respectively．Atthe time，the fragment ofBamH I-D eontniningthe chiA has been cloned．
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      Construction of Gene Library and Localization of Chitinase Gene of Parocneria orienta Nuclear polyhedrovirus

      • 1. 1．Institute of virology，Chinese Academy of Sciences，Wuhan 430071，China 2．Sichuan Academy of sciences，Chendu 610081，China

      Abstract: The gene library of Parocneria oriema nuclear polyhedlovims(PaorNPV)was panly constructed by doing$oiriefragnen~ ofBamH I and EcoR I ofP~rNPV genomic DNA into pGEM3Z(f)as a vector．Using the chifinase gene of Hear SNPV probed wlth[a-32p]dCTP，the chifinase gene ofPaorNPV localized OI3_the fragraents of BamH I-D，EcoR[-A，‰ dⅢ一A，Pst LD and Xho tG／H respectively．Atthe time，the fragment ofBamH I-D eontniningthe chiA has been cloned．

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