Citation: YAO Zhi, DONC Cuan-mu, YU Yong-xin, JU Ke, YAN Yu-ehe. Molecular Characterristics of the Seed Virus 0f Hl S Candidate Vaccine A16 Strain .VIROLOGICA SINICA, 2001, 16(4) : 315-320.

Molecular Characterristics of the Seed Virus 0f Hl S Candidate Vaccine A16 Strain

  • Available online: 05 December 2001
  • For further studying on the molecular characteristics of A16 strain,which is the seed virus of HFRS candidate vaccine,the M and S segments of A16 strain were amplified by RT PCR and then sequenced respectively.Sequence assay showed that Msegment of A16 strain contained 3615 nucleotides,encoding 1133 amino acids,and S segment of A16 strain contained 1 770 nucleotides,encoding 429 amino acids.The homology of M segment of A16 strain’s nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 75.7% to 90.3% and 85.9% to 97.1%respectively.Namely,the mutation of M segment of A16 strain’s nucleotide sequence and amino acid sequence with that of other HTN type virus varied from 9.7% to 24.3% and 2.9% to 14.1% respectivedly.Like M segment,the homology of S segment of A16 strain’s nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 76.0% to 90.0% and 92.1% to 97.7%,and 67.0% to 67.7% and 81.7% to 82.6% with that of SE

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    Molecular Characterristics of the Seed Virus 0f Hl S Candidate Vaccine A16 Strain

    • 1. I. National lnstitute for the Control of Pharmecetuical and Bidbgyical Products, Beijing l100050, China
    • 2. lnstitute of Epidemioldgy and Microbiology, Chinese Preventive Medical Academy of Science

    Abstract: For further studying on the molecular characteristics of A16 strain,which is the seed virus of HFRS candidate vaccine,the M and S segments of A16 strain were amplified by RT PCR and then sequenced respectively.Sequence assay showed that Msegment of A16 strain contained 3615 nucleotides,encoding 1133 amino acids,and S segment of A16 strain contained 1 770 nucleotides,encoding 429 amino acids.The homology of M segment of A16 strain’s nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 75.7% to 90.3% and 85.9% to 97.1%respectively.Namely,the mutation of M segment of A16 strain’s nucleotide sequence and amino acid sequence with that of other HTN type virus varied from 9.7% to 24.3% and 2.9% to 14.1% respectivedly.Like M segment,the homology of S segment of A16 strain’s nucleotide sequences and induced amino acid sequences with that of other HTN type virus varied from 76.0% to 90.0% and 92.1% to 97.7%,and 67.0% to 67.7% and 81.7% to 82.6% with that of SE

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