Citation: FAN Lingyun, HU Zhihong, Just.M.Vlak, CHEN Xinwen. Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene .VIROLOGICA SINICA, 2001, 16(4) : 344-349.

Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene

  • Available online: 05 December 2001
  • In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8~59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular mass of 146kDa.A late transcriptional motif,ATAAG,was found at 50nt upstream of the translational start codon ATG,while two TATA box was located at 112nt and 189nt upstream of ATG.A typical poly(A) signal was found at 12nt downstream of the translational stop codon.Compared HaSNPV helicase with the helicases of Autographa colifornica MNPV(AcMNPV),( Bombyx bori NPV (BmNPV), Lymantria dispar MNPV(LdMNPV) Spodoptera exigue MNPV (SeMNPV), Orgyia pseudotsugata MNPV (OpMNPV),and Xestia c nigrum granuloviurs(XcGV),only 5motifs(I,Ia,II,III,IV)were found conserved in baculovirus.The homology of the other two notifs (V and VI),which are also quite conserved in other helicases,were lowe

  • 加载中
  • 加载中

Article Metrics

Article views(4372) PDF downloads(868) Cited by()

Related
Proportional views

    Sequence Analysis of Helicoverpa armigera Single—nucleocapsid Nucleopolyhedrovirus heficase Gene

    • 1. 中国科学院武汉病毒研究所无脊椎动物病毒学联合实验室 湖北武汉 of Virology Laboratory Wageningen University Bennenhaven11 6709PD Walgen

    Abstract: In order to investigate the genomic organization of the single nucleocapid nucleopolyhedrovirus of Helicoverpa armigera ,the Eco R I N fragment located at 54.8~59.3 kbp of the viral genome was sequenced.The fragment contained 3 762 bp helicase gene potentially encoding a protein with a molecular mass of 146kDa.A late transcriptional motif,ATAAG,was found at 50nt upstream of the translational start codon ATG,while two TATA box was located at 112nt and 189nt upstream of ATG.A typical poly(A) signal was found at 12nt downstream of the translational stop codon.Compared HaSNPV helicase with the helicases of Autographa colifornica MNPV(AcMNPV),( Bombyx bori NPV (BmNPV), Lymantria dispar MNPV(LdMNPV) Spodoptera exigue MNPV (SeMNPV), Orgyia pseudotsugata MNPV (OpMNPV),and Xestia c nigrum granuloviurs(XcGV),only 5motifs(I,Ia,II,III,IV)were found conserved in baculovirus.The homology of the other two notifs (V and VI),which are also quite conserved in other helicases,were lowe

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return