Citation: Du Jian-yu, IANG Jia-rnin, GUO Hai-tao, ZHANG xiao-cto, . Molecular Cloning and Sequencing of Dendt~limuspunctatus Cytoplasmic Polyhedrosis Virus(DpCPV)oolyhedrin gene .VIROLOGICA SINICA, 2001, 16(4) : 350-354.

Molecular Cloning and Sequencing of Dendt~limuspunctatus Cytoplasmic Polyhedrosis Virus(DpCPV)oolyhedrin gene

  • Available online: 05 December 2001
  • CPVs belong to the genus Cypovirus in the family Reoviridae and infect midgut epithelial cells of the wide range of insects.For the synthesis of cDNA of Dendrolimus punctatus CPV (DpCPV) polyhedrin gene,the primers were constructed on the basis of the terminal RNA sequence of BmCPV segment 10,After RT PCR,the amplified cDNA was cloned into the pGEM T.The S10 cDNA of DpCPV was found to be 763 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 248 residues (Mr of 28411).Comparison of the nucleotide sequence of the polyhedrin gene of DpCPV with that of BmCPV showed that nucleotides homology is 89.3%,but there are only 6 amino acid differences between the two CPVs.

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    Molecular Cloning and Sequencing of Dendt~limuspunctatus Cytoplasmic Polyhedrosis Virus(DpCPV)oolyhedrin gene

    • 1. 1 1nstitute of Virology,wuhan University,Wuhan 430072,China:,2.Beijing Union Medical Academy, 100730,china

    Abstract: CPVs belong to the genus Cypovirus in the family Reoviridae and infect midgut epithelial cells of the wide range of insects.For the synthesis of cDNA of Dendrolimus punctatus CPV (DpCPV) polyhedrin gene,the primers were constructed on the basis of the terminal RNA sequence of BmCPV segment 10,After RT PCR,the amplified cDNA was cloned into the pGEM T.The S10 cDNA of DpCPV was found to be 763 nucleotides in length with a single open reading frame in one strand capable of coding a predicted protein of 248 residues (Mr of 28411).Comparison of the nucleotide sequence of the polyhedrin gene of DpCPV with that of BmCPV showed that nucleotides homology is 89.3%,but there are only 6 amino acid differences between the two CPVs.

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