Citation: HU Rong, MENG Xiao-Lin, XU Jin, WANG Jian, LIJ Wei. Expression of 2.6kb Enhancin Gene Fragment from Helicoverpa atmigera Granulosis Virus .VIROLOGICA SINICA, 2001, 16(4) : 364-368.

Expression of 2.6kb Enhancin Gene Fragment from Helicoverpa atmigera Granulosis Virus

  • Available online: 05 December 2001
  • The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression product(P 102 ) on Helicoverpa armigera nuclear polyhedrosis virus(HaNPV)and Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%~27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.

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    Expression of 2.6kb Enhancin Gene Fragment from Helicoverpa atmigera Granulosis Virus

    • 1. The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression productP 102 on Helicoverpa armigera nuclear polyhedrosis virusHaNPVand Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%~27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.

    Abstract: The 2.6kb fragment of enhancing gene from Helicoverpa armigera granulosis virus was inserted into vector pQE32 and expressed successfully in E.coli M15.The synergy of expression product(P 102 ) on Helicoverpa armigera nuclear polyhedrosis virus(HaNPV)and Bt against the 2nd instar larvae of H.armigera was studied.The results indicated that the accumulated mortality of the larvae increased 6.25%~27.09% on 168h post infection and the median lethal time decrease at least 12.3h in HaNPV+En treated compared with those in HaNPV treated.The accumulated mortality of the larvae increase 28.18% on 72h postinfection and the median lethal time decreased 12.33h in Bt+En treated compared with that in Bt treated.

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