Citation: CHANG Guo-hui.CHEN Sheng—hang“, LUO Ban-jun, u Tian-xia, . Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus .VIROLOGICA SINICA, 2001, 16(4) : 390-392.

Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus

  • Available online: 05 December 2001
  • The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.

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    Location and Clone of the Polyhedrin Gene of Ecproctis pseudoconspera Nuclear Polyedrosis Virus

    • 1. 慨血mlnsdace o厂V/rdogy,Ch/r~e缸I sc~nce*,Wuhan 430071.0

    Abstract: The polyhedrin gene of EpNPV was located on Hin d III E, Xba I B, Eco RI J, Kpn I A, Pst I F, Bam H I G fragments under stringent condition(68℃,in water),by using the DIG labeled Bam H I F fragment of AcMNPV DNA as the probe.In order to sequence the EpNPV polyhedrin gene,the Bam H I G fragment was cloned into pTZ19R vector.Then the fragment was digested by Xba I? Hin d III,and subcloned into pTZ19R.

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