Cloning and Sequence Analysis of Buchnera GroEL Genefrom Endosybiotic Bacterium of M yzus persicae

Buchnera groEL gene of endosymbiotic bacterium (Buc hnera sp.) from Myzus persicae Yangling boitype (P.R.China) was isloated,a n d the complete nucleotides sequence was determined.It is indicated that Buchne ra groEL gene of M.persicae includes 1647 base pairs,has 99% and 91% sequ ce similarity with Buchnera groEL gene of M.persicae The Nether land bi otype (AF003957) and Acyrthosiphon pisum Japanese biotype (X61150),respectiv ely.Buchnera GroEL-YL contains 548 amino acide,and has 3 different amino ac ids comparing with Buchnera GroEL-NT,that is AA111MetLys、AA222ValMet and AA348GlnHis.