The Studies on Expression of 1 197 Gene of Prawn Baculovirus and on Purifications of the Expressed Product

In previous report we have cloned a putative early and late transcriptional gene 1 197 of prawn baculovirus．In order to detect the biological function of this gene ，1197 gene was inserted into expressing vector pGEX-3X．The fusion protein expressed with high yield gua$obtained，but it~1)as foundthattheAsion proteinlocatesininclusion bodies ofE．coli andit causod troubleinitspurfica— tion．Wefoundthatthe expressed productwithM ．of 66kD could bepurifiedfrom inclusion hod— ies by using a serious of treatments of inclusion bodies r~,ith ug’ea and guanidine hydrochloride and then by sequential FPLC chromatography．