Genetic Organization of the EcoR I-G Region of the Spodoptera litura Nucleopolyhedrovirus

In order to investigate the genomic organzation of the S／,o’ptera litura multinucleoeapsid nucleopolyhedrovirus(SphNPV)，the EcoR 1-G[ragment located at map units(mu)49 2-54．1 ol the viral genome was sequenced．The fragment contains 2 partial and 5 complete open reading frames (ORF)representing the viral capsid protein gene p 39 and P 91，[ate expression factor 4(z 4)， cg30 gene，tlp20 gene，including homologous region(hr)+Sequence analysis further revealed that these ORFs have the same conserved motifs and gene structure as those observed in 【heir homologues from other baculovirus do．The genomic arrangement of the ORFs in the SpltNPV is similar in aI1 the bac— oloviirus genome，it maybe reveals that this fragment is the conserved part in the baculovirus