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Volume 17 Issue 2
April 2002
    Citation: WANG Zhanghui, ZHOU Yi-jun, FAN Yong jian．CHENG Zhao-bang, ZHANG Wen—hui, . cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu .VIROLOGICA SINICA, 2002, 17(2) : 142-144.
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    cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu

    • 1.

      Jiangsu Academy Agricultural Sciences,Nanjing 210014．China

    • Available online: 05 April 2002
    • An isolate of rice black-streaked dwarf Fijivirus originally from Lianyuangng，Jiangsu，China was used tO inoculated maize tO propagate the virus Improved single primer amplification technique was adopted tO clone the vira1 genomic dsRNA SIO for sequence determination The result showed：the full length of S10 is 1 801 bp and has the same organization with M RDV in Italy and RBSDV in Japan The similarity of nucleotide and predicted protein weDe 87 5％ and 92．6％ with MRDV． 93．3％ and 96．4％ with RBSDV ．This study provides a new methodforthe sequence determination of viral dsRNA
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      cDNA Clone and Sequence Analysis of Segment 10 of Rice Black．Streaked Dwarf Fijivirus in Jiangsu

      • 1. Jiangsu Academy Agricultural Sciences,Nanjing 210014．China

      Abstract: An isolate of rice black-streaked dwarf Fijivirus originally from Lianyuangng，Jiangsu，China was used tO inoculated maize tO propagate the virus Improved single primer amplification technique was adopted tO clone the vira1 genomic dsRNA SIO for sequence determination The result showed：the full length of S10 is 1 801 bp and has the same organization with M RDV in Italy and RBSDV in Japan The similarity of nucleotide and predicted protein weDe 87 5％ and 92．6％ with MRDV． 93．3％ and 96．4％ with RBSDV ．This study provides a new methodforthe sequence determination of viral dsRNA

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