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Volume 17 Issue 2
April 2002
    Citation: JIANG Yan ．HOU Yu—feng, CHEN De—sheng, CHEN Pu yan “, . Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain .VIROLOGICA SINICA, 2002, 17(2) : 149-152.
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    Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain

    • 1.

      1．College of Veterinary Medicine，Nanjing Ag iculture University，Nanjing 210095，China

    • 2.

      Nanjin Entry-Entry lnspection and quranting Beaury Nanjing 210001 China

    • Available online: 05 April 2002
    • According to the Genbank open sequence，two pairs of primer were designed in order to am — plify gE gene and partial gI gene of pseudorabies virus Shanghai strain，respectively．The g￡gene and partial gI gene were obtained by po]ymerase chain reaction(PCR)，subsequently cloned into pGEM T vector．Then both of them were inserted into pUCI8 vector．The recombinant plasmid pgEI deleted part of gI gene was constructed．
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      Cloning，Construction of gE Gene and gI Gene Partial Deletion Mutant of Pseud0rabies Virus SH Strain

      • 1. 1．College of Veterinary Medicine，Nanjing Ag iculture University，Nanjing 210095，China
      • 2. Nanjin Entry-Entry lnspection and quranting Beaury Nanjing 210001 China

      Abstract: According to the Genbank open sequence，two pairs of primer were designed in order to am — plify gE gene and partial gI gene of pseudorabies virus Shanghai strain，respectively．The g￡gene and partial gI gene were obtained by po]ymerase chain reaction(PCR)，subsequently cloned into pGEM T vector．Then both of them were inserted into pUCI8 vector．The recombinant plasmid pgEI deleted part of gI gene was constructed．

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