四株草鱼呼肠孤病毒毒株的细胞感染特性比较研究

Some characterizations of four Grass Carp Reovirus (GCRV) strains were comparatively studied at the first time. It showed that the stored GCRV\-\{873\},GCRV\-\{875\} and GCRV\-\{876\} at minus 30℃ for ten years still remained infectious to CIK cells, and the 3 virus titers could reach above 10\+2 TCID\-\{50\}/mL, which was a little lower than the newly isolated GCRV\-\{991\} strain′s. After inoculation 3-4 generations, their titers rised gradually, and the titer of GCRV\-\{873\} could be up to the highest, which was almost 6.4×10\+\{11\} TCID\-\{50\}/mL with 0.05PFU/cell multiplicity of infection (MOI) at 28℃. The infective ability of the four strains at different temperatures (28℃,31℃,34℃,37℃ and 41℃) was also detected, which revealed that CPE appeared in CIK cells under above conditions, but titers depressed as the temperature rised. In addition, 4 GCRV-ds RNAs were also compared in a same electronic condition