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Volume 17 Issue 2
April 2002
    Citation: SUN Xue-qiang, LU Cheng-ping. Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs .VIROLOGICA SINICA, 2002, 17(2) : 185-188.
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    Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

    • 1.

      Kay lab of Aniatat Disease Diagnosis＆ tmmunotogy，Ministry of Agriculture，Nanjing Agri Univ，Nanjing 210095，China

    • Available online: 05 April 2002
    • Abstract：The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho— mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by gel filtration chromatogmph with Sepia,arose 4B．The eluates collected were concentrated at 4℃ ．The virus particles in the first part of the eluates could be seen under electron microscopy．The diameter of virions varified jn 50～ 100nm Sinopotamon denticu缸￡“?II artilicia】inlected with the purified virus became j1]．Bv double antibody sandwich EI ISA detection．the results showed that there was signifi— cai]t diflerence between the jnfected and the centre crabs
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      Isolation and Purification of the Eriocheir sinensis “Tremble Disease”Virus and Its Pathongenity on Crabs

      • 1. Kay lab of Aniatat Disease Diagnosis＆ tmmunotogy，Ministry of Agriculture，Nanjing Agri Univ，Nanjing 210095，China

      Abstract: Abstract：The Eriocheir sinensis(Es)that suffered“tremble diease”by artificial infection were ho— mogenated The supernatant was concentrated with p。】yethyIeneg1ycol(PEG)6000 and purified by gel filtration chromatogmph with Sepia,arose 4B．The eluates collected were concentrated at 4℃ ．The virus particles in the first part of the eluates could be seen under electron microscopy．The diameter of virions varified jn 50～ 100nm Sinopotamon denticu缸￡“?II artilicia】inlected with the purified virus became j1]．Bv double antibody sandwich EI ISA detection．the results showed that there was signifi— cai]t diflerence between the jnfected and the centre crabs

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