Citation: XIONG Li—juan, LUO Duan—de, ZENG Ling—lan, CAI Shu—qing. Study on the Endonuelease Site of G1 Region of Hantavirus Genome .VIROLOGICA SINICA, 2002, 17(3) : 195-197.

Study on the Endonuelease Site of G1 Region of Hantavirus Genome

  • Available online: 05 June 2002
  • Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of 34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan.The products contained within G 1 gene of M segm ent, w ere digested with H in C II and Sac I respectively, thereby, han— tavirus could be typed into HTN and SEO.The results of RFLP—PCR were highly consistent with typ— ing RT—PCR,and furthermore,RFI P—PCR could quickly screen the variant strain with gene mutation at the restriction site of HiI"l C II or SacI.

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    Study on the Endonuelease Site of G1 Region of Hantavirus Genome

    • 1. Department of Infectious Diseases,Union Hospital,Tong.ji Medical College, Huazhong University of Technology and Science.Wuhan 430022,China

    Abstract: Typing RT—PCR was used to amplify hantaviruses genome from peripheral blood samples of 34 patients with hemorrhagic fever with renal syndrom e hospitalized in W uhan.The products contained within G 1 gene of M segm ent, w ere digested with H in C II and Sac I respectively, thereby, han— tavirus could be typed into HTN and SEO.The results of RFLP—PCR were highly consistent with typ— ing RT—PCR,and furthermore,RFI P—PCR could quickly screen the variant strain with gene mutation at the restriction site of HiI"l C II or SacI.

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