Citation: Li Xin, YANG Wei, HANG Chang, BAI Xue, MA Ben, H UANG, LI Guang, ZHA NG. Sequence Analysis of Com plete L Segm ent of 84FLi, a Hantann Virus Isolated from Xi’an .VIROLOGICA SINICA, 2002, 17(3) : 221-225.

Sequence Analysis of Com plete L Segm ent of 84FLi, a Hantann Virus Isolated from Xi’an

  • Available online: 05 June 2002
  • In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i,the cDNA of L segment was amplified fragment by fragment by RT—PCR .The purified PCR products were sequenced directly or cloned into pM D18一T vector and then sequenced.The I genom e segm ent is 6533 nucleotides in length with a predicated region(from 38 to 6493)encoding 2151 amino acids.The entire sequence com position was A 33.39% ,C16.43% ,G20.74% ,T29.44% ,and the GC and AT contents were 37.17 and 62.83% .Homology analysis showed that the homologies of 84FLi L segment nu— cleotide sequence with HTN isolates especially Chinese isolates were higher than those of other Han— tavirus.It showed 83.7% homology with HTN foreign standard strain 76—118。while the amino acid homology between the two strains was 97.5% .The result suggested that 84FLi strain was one of the Hantann virus,and highly related tO other Chinese Hantaan Virus iso lates.

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    Sequence Analysis of Com plete L Segm ent of 84FLi, a Hantann Virus Isolated from Xi’an

    • 1. 1.Infectious Diseases Center of P.L.A,Tangdu Ho tal,Fourth Military Medical University,Xi’an 710038,China
    • 2. Institute of Virology,Chinese Academy of Preventive Medicine,Beijing 100052。Ch ina

    Abstract: In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i,the cDNA of L segment was amplified fragment by fragment by RT—PCR .The purified PCR products were sequenced directly or cloned into pM D18一T vector and then sequenced.The I genom e segm ent is 6533 nucleotides in length with a predicated region(from 38 to 6493)encoding 2151 amino acids.The entire sequence com position was A 33.39% ,C16.43% ,G20.74% ,T29.44% ,and the GC and AT contents were 37.17 and 62.83% .Homology analysis showed that the homologies of 84FLi L segment nu— cleotide sequence with HTN isolates especially Chinese isolates were higher than those of other Han— tavirus.It showed 83.7% homology with HTN foreign standard strain 76—118。while the amino acid homology between the two strains was 97.5% .The result suggested that 84FLi strain was one of the Hantann virus,and highly related tO other Chinese Hantaan Virus iso lates.

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