Sequence Analysis of Com plete L Segm ent of 84FLi, a Hantann Virus Isolated from Xi’an
Abstract: In order tO study the nucleotide sequence of L segment of Hantaan virus strain 84FI i,the cDNA of L segment was amplified fragment by fragment by RT—PCR .The purified PCR products were sequenced directly or cloned into pM D18一T vector and then sequenced.The I genom e segm ent is 6533 nucleotides in length with a predicated region(from 38 to 6493)encoding 2151 amino acids.The entire sequence com position was A 33.39% ,C16.43% ,G20.74% ,T29.44% ,and the GC and AT contents were 37.17 and 62.83% .Homology analysis showed that the homologies of 84FLi L segment nu— cleotide sequence with HTN isolates especially Chinese isolates were higher than those of other Han— tavirus.It showed 83.7% homology with HTN foreign standard strain 76—118。while the amino acid homology between the two strains was 97.5% .The result suggested that 84FLi strain was one of the Hantann virus,and highly related tO other Chinese Hantaan Virus iso lates.