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Volume 17 Issue 3
June 2002
    Citation: HUANG Ya, ZHEN Qing, WANG Lin, LI Xiao-kun, HUANG Zi. Cloning and Determ ination of S 1 Gene of IBV GD05 Strain and Its Expression in E ．coli． .VIROLOGICA SINICA, 2002, 17(3) : 266-269.
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    Cloning and Determ ination of S 1 Gene of IBV GD05 Strain and Its Expression in E ．coli．

    • 1.

      1．Biopharmaceutic R&D center of Jinan University，Guangzhou 510632，China

    • 2.

      Department of veterinary medicine South china Agriculture University，@uangzhou 510642，China

    • 3.

      Depa rtment of Sericulture， South china Ag riculture University，Guangzhou 510642，China

    • Available online: 05 June 2002
    • Acording tO Avian Infectious Brochitis virus(IBV)Beaudette strain S 1 gene sequence，a pair of primers were designed and synthesized．W ith the primers， IBV Guangdong isolation strain GD05 S 1 gene was successively amplified by RT — PCR ．PCR product was digested w ith BstY I，Hae III and Pst I respectively，the result showed RFLP pattern of was the same as that of M41 S 1 gene． IBV GD05 strain was thought as M ass serotype primaryly．IBV GD05 S 1 gene was cloned into pGEM — T vector and sequenced， its sequence was consisted of 1611 base pairs．By comparison ， the nu— cleotide sequence was 97．14％ identical tO that of IBV M 41． IBV GD05 S 1 gene was subcloned into expression vector pET21 d．SDS—PAGE experiment showed that it expresed in E ．coli．
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      Cloning and Determ ination of S 1 Gene of IBV GD05 Strain and Its Expression in E ．coli．

      • 1. 1．Biopharmaceutic R&D center of Jinan University，Guangzhou 510632，China
      • 2. Department of veterinary medicine South china Agriculture University，@uangzhou 510642，China
      • 3. Depa rtment of Sericulture， South china Ag riculture University，Guangzhou 510642，China

      Abstract: Acording tO Avian Infectious Brochitis virus(IBV)Beaudette strain S 1 gene sequence，a pair of primers were designed and synthesized．W ith the primers， IBV Guangdong isolation strain GD05 S 1 gene was successively amplified by RT — PCR ．PCR product was digested w ith BstY I，Hae III and Pst I respectively，the result showed RFLP pattern of was the same as that of M41 S 1 gene． IBV GD05 strain was thought as M ass serotype primaryly．IBV GD05 S 1 gene was cloned into pGEM — T vector and sequenced， its sequence was consisted of 1611 base pairs．By comparison ， the nu— cleotide sequence was 97．14％ identical tO that of IBV M 41． IBV GD05 S 1 gene was subcloned into expression vector pET21 d．SDS—PAGE experiment showed that it expresed in E ．coli．

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