Citation: WU Dong, W ANG Hua—lin, DENG Fei, CHEN Xin—wen, PENG Hui—yin。HU Zhi—hong, . Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli .VIROLOGICA SINICA, 2002, 17(4) : 331-335.

Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli

  • Available online: 05 November 2002
  • The PCR product of the HaSNPV chitinase gene,which was without the N—terminal signal peptide and the C-terminal endoplasmic reticulum location signal,was cloned into a prokaryotic expres— sion vector pProEXHTb.After the induction of IPTG,the chitinase gene was successfully expressed in Eschechia coli DH5a.The expression product shown a molecular weight of 60 kDa,and it covered about 40% of the total protein in E .coli.W estern blot analysis using an antibody derived from AcM — NPV ehitinase confirm ed the expression product was a homologue of baculoviral chitinase.

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    Molecular Cloning and Expression of the Chitinase Gene from Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus in E.coli

    • 1. Joint—lab of Invertebrate Virology,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071.China

    Abstract: The PCR product of the HaSNPV chitinase gene,which was without the N—terminal signal peptide and the C-terminal endoplasmic reticulum location signal,was cloned into a prokaryotic expres— sion vector pProEXHTb.After the induction of IPTG,the chitinase gene was successfully expressed in Eschechia coli DH5a.The expression product shown a molecular weight of 60 kDa,and it covered about 40% of the total protein in E .coli.W estern blot analysis using an antibody derived from AcM — NPV ehitinase confirm ed the expression product was a homologue of baculoviral chitinase.

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