Citation: FU Ming—jia, WU Zu—jian, LIN Qi—ying’’, XIE Lian—hui. Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus .VIROLOGICA SINICA, 2002, 17(4) : 350-353.

Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus

  • Available online: 05 November 2002
  • A antiviral protein。YP46—46,was isolated and purified to homogeneity from the mushroom, Plearotus citrinopileatus.by precipitation of 40% 一60% saturation of ammonium sulfate followed by DEAE-sepharose FF ion—exchange column chromatography。 Sephacryl M S一200 High Resolution molecular sieve chromatography.The protein has a molecular weight of about 27.4kD by SDS-PAGE. The concentration of the protein was 0.24/~g/mL when the inhibition rate against Tobacco mosaic irus was 50% .Using an assay system based on HepG2.2.2.1 5 cell line,YP46—46 was studied for its inhibitory ability against Hepatitis B virus.The result shows that the concentration of 50% inhibition of HBsAg was 0.08ttg/mL,but low effect on HBeAg.

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    Purification of a Antiviral Protein in Plearotus citrinopileatus and Its Activities against Tobacco mosaic virus and Hepatitis B virus

    • 1. Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou 350002,China

    Abstract: A antiviral protein。YP46—46,was isolated and purified to homogeneity from the mushroom, Plearotus citrinopileatus.by precipitation of 40% 一60% saturation of ammonium sulfate followed by DEAE-sepharose FF ion—exchange column chromatography。 Sephacryl M S一200 High Resolution molecular sieve chromatography.The protein has a molecular weight of about 27.4kD by SDS-PAGE. The concentration of the protein was 0.24/~g/mL when the inhibition rate against Tobacco mosaic irus was 50% .Using an assay system based on HepG2.2.2.1 5 cell line,YP46—46 was studied for its inhibitory ability against Hepatitis B virus.The result shows that the concentration of 50% inhibition of HBsAg was 0.08ttg/mL,but low effect on HBeAg.

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