Amplification and Clone of VP2-4-3 Gene of Very Virulent Infectious Bursal disease virus
Abstract: The methods of reverse transcription,polymerase chain reaction(PCR)amplification,and cloning of full—length VP2—4—3 gene of a very virulent infectious Bursal disease virus(vvlBDV)strain SH95 were developed.The use of random primer and a reverse transcriptase lacking RNase—H activity produced full—length coding region and non—coding region eDNA copies of the viral genomic segments. The 3060 base—pairs(bp)of VP2—4—3 were amplified by long and accurate PCR in a single step,SUC— cessfully cloned and sequenced revealing their identity of IBDV.