Citation: SUN Jian—he, JIANG Jing, LU Ping, ZHAO Yu. Amplification and Clone of VP2-4-3 Gene of Very Virulent Infectious Bursal disease virus .VIROLOGICA SINICA, 2002, 17(4) : 358-361.

Amplification and Clone of VP2-4-3 Gene of Very Virulent Infectious Bursal disease virus

  • Available online: 05 November 2002
  • The methods of reverse transcription,polymerase chain reaction(PCR)amplification,and cloning of full—length VP2—4—3 gene of a very virulent infectious Bursal disease virus(vvlBDV)strain SH95 were developed.The use of random primer and a reverse transcriptase lacking RNase—H activity produced full—length coding region and non—coding region eDNA copies of the viral genomic segments. The 3060 base—pairs(bp)of VP2—4—3 were amplified by long and accurate PCR in a single step,SUC— cessfully cloned and sequenced revealing their identity of IBDV.

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    Amplification and Clone of VP2-4-3 Gene of Very Virulent Infectious Bursal disease virus

    • 1. The Institute of Bio Technology ,School of Agriculture,Shanghai JiaoTong University,Shanghai,201101,China

    Abstract: The methods of reverse transcription,polymerase chain reaction(PCR)amplification,and cloning of full—length VP2—4—3 gene of a very virulent infectious Bursal disease virus(vvlBDV)strain SH95 were developed.The use of random primer and a reverse transcriptase lacking RNase—H activity produced full—length coding region and non—coding region eDNA copies of the viral genomic segments. The 3060 base—pairs(bp)of VP2—4—3 were amplified by long and accurate PCR in a single step,SUC— cessfully cloned and sequenced revealing their identity of IBDV.

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