Citation: CHENG Zi—qiang, ZHAO Zhen—hua, HAO Yong—qing, ZHAO Xin—li, H ASI Agual. Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis .VIROLOGICA SINICA, 2002, 17(4) : 371-373.

Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

  • Available online: 05 November 2002
  • We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region.The result indicated that the meat—type flocks of three dif—ferent farms infection rate'.of ALV—J were 28.12%(9/32)、9.38%(3/32)and 8.33%(10/12),respec—tively,and all laying chickens infection rate were 0%(0/35,0/10).We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test.The result indicated that6 chickens of ALV—J antibody positive were all PCR positive,and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative.The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens.

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    Serology Investigatio and PCR Diagnosis of Avian M yelocytomatosis

    • 1. 1.Institute ofAnimal Science and Animal Medicine,ShanDong Agriculture University,TaiAn 271018
    • 2. Institute of Animal Science and Animal Medicine,Inner Mongolia Agriculture University,Huhhot 010018,China
    • 3. Examination Croup of Inner Mongolia Veterinary Station,Huhhot 010020

    Abstract: We investigated the infection of Avian leukosis virus subgroup J myelocytomatosis usingEI ISA method in Inner Mongolia Region.The result indicated that the meat—type flocks of three dif—ferent farms infection rate'.of ALV—J were 28.12%(9/32)、9.38%(3/32)and 8.33%(10/12),respec—tively,and all laying chickens infection rate were 0%(0/35,0/10).We chose 6 chickens of ALV—J an—tibody positive and 13 chickens of ALV—J antibody negative to do PCR test.The result indicated that6 chickens of ALV—J antibody positive were all PCR positive,and 4 chickens were PCR positive among1 3 chickens of AI V—J antibody negative.The result indicated that there were ALV—J virus not only inAI V—J antibody positive chickens but also in some AI V—J antibody negative chickens.

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