Citation: ZHANG Fang—lin, XU Zhi—kai, LUO Wen, YAN Yan, WU Xing—an, LIU Yong, BAI Wen—tao, ZHAO Qian, WANG Hai—ta. Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays .VIROLOGICA SINICA, 2003, 18(1) : 14-17.

Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays

  • Available online: 02 February 2003
  • Abstract:Recombinant euckaryotic expression vectors pcDNA3.1二G2S0.7 and pcDNA3.1一S0.7G2 were constructed by cloning chimeric genes containing G2 fragment of M segm ent and 0.7Kb fragment of S segment into pcDNA3.1 (+、. Then BALB/c mice were vaccinated by the two vectors. EUSA results showed that both the vectors could induce specific antibodies against NP and GP.The specific antibody titers stimulated by pcDNA3.1一G2S0.7 were obviously higher、that that of pcDNA3.1一S0.7G2. M IT re— sults showed that the stimulation indexes of splenocytes of pcDNA3.1一G2S0.7 to NP and GP were sig— nificantly higher than that of contro1.However.that of pcDNA3.1一SO.7G2 were equal to that of contro1. It is suggested that the chimeric genes of Hantaan virus containing G2 fragment of M segm ent and 0.7Kb fragment of S segm ent could directly specific anti—Hantaan virus humoral immunity and cellular immunity in BALB/c mice.Th e diferent splicing ways could afect the immunity of chimeric genes.

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    Studies on Genetic Immunization of the Chimeric Genes of Hantaan virus M and S Segments that Spliced by Diferent W ays

    • 1. Department ofMicrobiology,而urth Military Medical University,Xi’nn 710032,China

    Abstract: Abstract:Recombinant euckaryotic expression vectors pcDNA3.1二G2S0.7 and pcDNA3.1一S0.7G2 were constructed by cloning chimeric genes containing G2 fragment of M segm ent and 0.7Kb fragment of S segment into pcDNA3.1 (+、. Then BALB/c mice were vaccinated by the two vectors. EUSA results showed that both the vectors could induce specific antibodies against NP and GP.The specific antibody titers stimulated by pcDNA3.1一G2S0.7 were obviously higher、that that of pcDNA3.1一S0.7G2. M IT re— sults showed that the stimulation indexes of splenocytes of pcDNA3.1一G2S0.7 to NP and GP were sig— nificantly higher than that of contro1.However.that of pcDNA3.1一SO.7G2 were equal to that of contro1. It is suggested that the chimeric genes of Hantaan virus containing G2 fragment of M segm ent and 0.7Kb fragment of S segm ent could directly specific anti—Hantaan virus humoral immunity and cellular immunity in BALB/c mice.Th e diferent splicing ways could afect the immunity of chimeric genes.

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