Citation: YU Hong-chun, ZHENG Gui-ling, WANG Xiao-yun, LI Guo—xun, ZHAO Kui-jun. Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV .VIROLOGICA SINICA, 2003, 18(1) : 31-34.

Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

  • Available online: 02 February 2003
  • Abstract: A new cell line,designated NEAU—Ms一9803 12,was established from 24一hr—old Mythimna separata eggs and was passaged 201 times.The cells were heterogeneous in morphology with spherical and spindle—shaped cells.Th e population doubling time of the cell line aws 47.82h in 120h at 28~C. The cell line was highly susceptible to Mythimna separata nll~leal"polyhedrosis vu’us (MsNPV).When the cells were inoculated at a multiplicity of infection (m.o.i.)of 0.08TCID50/cell with wild pas— sage MsNPV。the infection rates and occlusion bodies f OB) yield reached a value of 62.10% , 33.59PIB/infected cell at 240h postinfection(pi),respectively

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    Establishment of a cell line from embryos of Mythimna separata and its susceptibility to M sNPV

    • 1. 1.Northeast Agricultural University,Harbin 150030,China.,2.Hebei Agricultural University,Baoding 071001,China

    Abstract: Abstract: A new cell line,designated NEAU—Ms一9803 12,was established from 24一hr—old Mythimna separata eggs and was passaged 201 times.The cells were heterogeneous in morphology with spherical and spindle—shaped cells.Th e population doubling time of the cell line aws 47.82h in 120h at 28~C. The cell line was highly susceptible to Mythimna separata nll~leal"polyhedrosis vu’us (MsNPV).When the cells were inoculated at a multiplicity of infection (m.o.i.)of 0.08TCID50/cell with wild pas— sage MsNPV。the infection rates and occlusion bodies f OB) yield reached a value of 62.10% , 33.59PIB/infected cell at 240h postinfection(pi),respectively

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