Serving the Society Since 1986
Advanced Search
Volume 18 Issue 1
February 2003
    Citation: WEN Li, ZHANG Jia—min, WANG Qiong, HU Jian-fang, HU Yuan-yang. M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus NS5 Protein Gene .VIROLOGICA SINICA, 2003, 18(1) : 49-53.
    shu

    M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus NS5 Protein Gene

    • 1.

      Institute of Virology。Wuhan University， uhan 430072，China

    • Available online: 02 February 2003
    • For the synthesis of cDNA of Dendrolimus punctaus CPV (DpCPV)NS5 protein，the primers were designed on the basis of the RNA sequmce of BmCPV一1(I strain)segment 9．After the RT—PCR the amplified cDNA was cloned into the pMD18一T． NS5 protein gene of DpCPV was found to be 963 nucleotides in length with a single open reading frame in one strand capable of coding a predicted pro— tein of 320 residues(Mw of 35．66kDa)． Comparison of the nucleotide sequences of the NS5 gene of DpCPV with those of BmCPV—I(1 strain)showed that nucleotides homology iS 86．0％ ， but there are only 2 1 amino acid differences between the two CPVs．
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(3836) PDF downloads(753) Cited by()

    Related
    Proportional views

      M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus NS5 Protein Gene

      • 1. Institute of Virology。Wuhan University， uhan 430072，China

      Abstract: For the synthesis of cDNA of Dendrolimus punctaus CPV (DpCPV)NS5 protein，the primers were designed on the basis of the RNA sequmce of BmCPV一1(I strain)segment 9．After the RT—PCR the amplified cDNA was cloned into the pMD18一T． NS5 protein gene of DpCPV was found to be 963 nucleotides in length with a single open reading frame in one strand capable of coding a predicted pro— tein of 320 residues(Mw of 35．66kDa)． Comparison of the nucleotide sequences of the NS5 gene of DpCPV with those of BmCPV—I(1 strain)showed that nucleotides homology iS 86．0％ ， but there are only 2 1 amino acid differences between the two CPVs．

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return