Citation: WEN Li, ZHANG Jia—min, WANG Qiong, HU Jian-fang, HU Yuan-yang. M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus NS5 Protein Gene .VIROLOGICA SINICA, 2003, 18(1) : 49-53.

M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus NS5 Protein Gene

  • Available online: 02 February 2003
  • For the synthesis of cDNA of Dendrolimus punctaus CPV (DpCPV)NS5 protein,the primers were designed on the basis of the RNA sequmce of BmCPV一1(I strain)segment 9.After the RT—PCR the amplified cDNA was cloned into the pMD18一T. NS5 protein gene of DpCPV was found to be 963 nucleotides in length with a single open reading frame in one strand capable of coding a predicted pro— tein of 320 residues(Mw of 35.66kDa). Comparison of the nucleotide sequences of the NS5 gene of DpCPV with those of BmCPV—I(1 strain)showed that nucleotides homology iS 86.0% , but there are only 2 1 amino acid differences between the two CPVs.

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    M olecular Cloning and Sequence Analysis of Dendrolimus punctatus cytoplasmic polyhedrosis virus NS5 Protein Gene

    • 1. Institute of Virology。Wuhan University, uhan 430072,China

    Abstract: For the synthesis of cDNA of Dendrolimus punctaus CPV (DpCPV)NS5 protein,the primers were designed on the basis of the RNA sequmce of BmCPV一1(I strain)segment 9.After the RT—PCR the amplified cDNA was cloned into the pMD18一T. NS5 protein gene of DpCPV was found to be 963 nucleotides in length with a single open reading frame in one strand capable of coding a predicted pro— tein of 320 residues(Mw of 35.66kDa). Comparison of the nucleotide sequences of the NS5 gene of DpCPV with those of BmCPV—I(1 strain)showed that nucleotides homology iS 86.0% , but there are only 2 1 amino acid differences between the two CPVs.

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