Cloning and Prokaryotic Expression of groEL Gene of Endosybiotic Bacterium from rhopalosiphum maize

Cloned one virus binding protein from Rhopalosiphum maize Yangling biotype through PCR technique，using designed specific primers based on this protein partially sequence．In nucleotide and amino acid sequeneee levels． this protein was identified as GroEL protein of endosymbiotic bacterium (Buchnera sp．) of aphid．The results of nucleotides sequencing indicated that the full length of groEL genes f GeneBank accessio number iS AF3 87863) iS 1 647bp and code 548 amino acids．Th e cloned groEL genes of and Rhopalosiphum maize were ligated into pBV22 1，pET30a and pTrcHisA vector for expressing The results suggested that 63 KDa aim proteins can be espressed using pBV22 1．When pET一30a used，the expressed fusion proteins have a molecular weight of 69 KDa，and both have a higher expression quantiy