Citation: WANG Yong-shan, Zhou Zong-an, DENG Xiao-zhao, ZHAO Xin-tai, HOU Ji-bo, HE Kong-wang, DING Chan, LIU Yu, GAO Jian, DIAO Zhen-yu. Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species .VIROLOGICA SINICA, 2003, 18(1) : 68-71.

Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species

  • Available online: 02 February 2003
  • Abstract:According to the published cDNA sequences of Infectious bursal disease virus(IBDV),a pairof primers to variable region in VP2 were designed for cDNA synthesis and PCR amplification of fourIBDV isolated from different bird species,CH,DU,GE and SP.The nucleotide sequences determinedhave been compared to each other.Th e four isolates are closely related,the homology of the four iso—lates being 97% at the nucleotied level and 98% at the amino acid leve1.Th eir two hydrophilic regionsand one heptapeptide were identical at the amino acid leve1. Th e results revealed that the ducks,geeseand sparrows naturally infected with IBDV not only could become the carriers or reservoirs in transmis—sion but may take certain effect on the variation of IBDV.

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    Analysis of the Sequence of Variable Region in vp2 of Infectiousbursal disease virus Isolated from Diferent Bird Species

    • 1. 1.HuadongResearchInstitute ofMedicalBiotechnics,Nanjing210002,China
    • 2. NationalLaboratoryforOncogenes andRelatedGenes,.Shanghai Cancer Institute,Shanghai 200032,China
    • 3. Institute ofVeterinaryScience,JiangsuAcademyofAgricultural Sciences,Nanjing210014,China
    • 4. Jiangsu Institute ofPoultry Science,Yangzhou 225003,China

    Abstract: Abstract:According to the published cDNA sequences of Infectious bursal disease virus(IBDV),a pairof primers to variable region in VP2 were designed for cDNA synthesis and PCR amplification of fourIBDV isolated from different bird species,CH,DU,GE and SP.The nucleotide sequences determinedhave been compared to each other.Th e four isolates are closely related,the homology of the four iso—lates being 97% at the nucleotied level and 98% at the amino acid leve1.Th eir two hydrophilic regionsand one heptapeptide were identical at the amino acid leve1. Th e results revealed that the ducks,geeseand sparrows naturally infected with IBDV not only could become the carriers or reservoirs in transmis—sion but may take certain effect on the variation of IBDV.

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