Citation: ZHOU Bin.CHAO Rui—bing, LU Yin—hua, CHEN De—sheng.CHEN Pu-yang*, . Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus .VIROLOGICA SINICA, 2003, 18(2) : 137-140.

Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus

  • Available online: 15 April 2003
  • While observing Duck viral hepatitis seed virus in chicken embryonic allantochoroin liquid. another symmetry nonenveloped icosahedral viron with a diam eter of 7O tO 80 am was found.In order to investigation this contam inating virus,we selected four random primers to am plify this unknown virus by RT—PCR method an d PCR method respectively,that resulted three DNA fragments,showing 99.5% ,99.6% an d 99.5% homologue respectively with the genome of Adenovirus type I,or Chicken embryo orphan lethal virus(CELOV)during the sequencing analysis(one cycle reaction)for them. This results suggested that Duck viral hepatitis seed virus was contam inated by CELOV

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    Amplification with Random PCR method and Researches for the DNA Fragment of the genome of Chicken emb~o lethal orphan virus

    • 1. Key Laboratory of Animal Disease Diagnostic& Immunology,Ministry of Agriculture ofPeople’Republic of China, NanjingAgricultural University,Nanjing 210095,China

    Abstract: While observing Duck viral hepatitis seed virus in chicken embryonic allantochoroin liquid. another symmetry nonenveloped icosahedral viron with a diam eter of 7O tO 80 am was found.In order to investigation this contam inating virus,we selected four random primers to am plify this unknown virus by RT—PCR method an d PCR method respectively,that resulted three DNA fragments,showing 99.5% ,99.6% an d 99.5% homologue respectively with the genome of Adenovirus type I,or Chicken embryo orphan lethal virus(CELOV)during the sequencing analysis(one cycle reaction)for them. This results suggested that Duck viral hepatitis seed virus was contam inated by CELOV

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