Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application*
Abstract: Abstract:In this experiment the conditions for the expressing of HCLV E2 glycoprotein A and D an tigenic domains in E coli were optimized.On this basis,the expressed protein was purified by dissolving the inclusion body in Tris.HC1 with 6 mol/L guan dinine an d passed throh a His Bind affinity chromatography.The product was collected an d th e concentrated recombinan t protein was quentified. Th e recombinan t protein was then used to coat 96一well ELISA plates an d applied to detect the titers of the serums from swine immunized with HCLV vaccine.