Citation: SU Xiao-yun, M IAO, XU Lihua, CHEN Desheng, CHEN Puyan. Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application* .VIROLOGICA SINICA, 2003, 18(2) : 164-168.

Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application*

  • Available online: 15 April 2003
  • Abstract:In this experiment the conditions for the expressing of HCLV E2 glycoprotein A and D an tigenic domains in E coli were optimized.On this basis,the expressed protein was purified by dissolving the inclusion body in Tris.HC1 with 6 mol/L guan dinine an d passed throh a His Bind affinity chromatography.The product was collected an d th e concentrated recombinan t protein was quentified. Th e recombinan t protein was then used to coat 96一well ELISA plates an d applied to detect the titers of the serums from swine immunized with HCLV vaccine.

  • 加载中
  • 加载中

Article Metrics

Article views(3882) PDF downloads(924) Cited by()

Related
Proportional views

    Expression and purification of HCLV E2 protein A,D antigenic domains and its preliminary application*

    • 1. I Key lab ofAnimal Diagnostic and Immunology,Ministry ofAgriculture,Nanjing Agricultural University,Nanfing 210095,China

    Abstract: Abstract:In this experiment the conditions for the expressing of HCLV E2 glycoprotein A and D an tigenic domains in E coli were optimized.On this basis,the expressed protein was purified by dissolving the inclusion body in Tris.HC1 with 6 mol/L guan dinine an d passed throh a His Bind affinity chromatography.The product was collected an d th e concentrated recombinan t protein was quentified. Th e recombinan t protein was then used to coat 96一well ELISA plates an d applied to detect the titers of the serums from swine immunized with HCLV vaccine.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return