cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus

Abstract：Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag extracted an d S4 was separated．RT—PCR amplification technique Wag adopted to clone the viral genomic dsRNA S4 flor sequence determination．The results showed：the full length of S4 is 3262bp，it contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length．Tlle similarity of predicted protein were 94％ 、92％ an d 22％ with LdCPV、BmCPV an d RRSV respectively． Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs were also present in the deduced protein．