cDNA Clone and Sequence Analysis of Segment 4 of Dendrolimus punctatus cytoplasmic polyhedrosis virus
Abstract: Abstract:Dendrolimus punctatus cytoplasmic polyhedrosis virus(DpCPV)genome dsRNA wag extracted an d S4 was separated.RT—PCR amplification technique Wag adopted to clone the viral genomic dsRNA S4 flor sequence determination.The results showed:the full length of S4 is 3262bp,it contains one large open reading fram e an d encodes a polype ptide with 1058 amino acids in length.Tlle similarity of predicted protein were 94% 、92% an d 22% with LdCPV、BmCPV an d RRSV respectively. Amino acid sequences simi lar to a GTPase、methyltran sferase of Methanosarcina mazef Goel motifs were also present in the deduced protein.