Citation: LIU De, DUAN Yuan, . Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene .VIROLOGICA SINICA, 2003, 18(3) : 254-258.

Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene

  • Available online: 18 June 2003
  • Abstract:A recombinant virus containing double copies of V—cath gene was constructed by using Han pvid Bac—to—Bac system.ThiS recombinan t virus was named as dciHaNP、 because it was inserted an other copy of V-cath gene controlled by iel promoter.Dot blot an d Northern blot an alysis showed that this recombinan t virus was correct.The toxicity was measured by using Hz—AM l cells infec ted with the recombinan t virus.Th e TCID5n ofdciHaNPlV is 3.16× l0 TCID5o/mL.

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    Construction and Toxicity Analysis of a Recombinant Viru s Contained Double Copies of v-cath Gene

    • 1. College of Life Science,Central China Normal University,Wuhan 430079,China

    Abstract: Abstract:A recombinant virus containing double copies of V—cath gene was constructed by using Han pvid Bac—to—Bac system.ThiS recombinan t virus was named as dciHaNP、 because it was inserted an other copy of V-cath gene controlled by iel promoter.Dot blot an d Northern blot an alysis showed that this recombinan t virus was correct.The toxicity was measured by using Hz—AM l cells infec ted with the recombinan t virus.Th e TCID5n ofdciHaNPlV is 3.16× l0 TCID5o/mL.

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