Citation: XU zhen, YING Song, SHEN Chao, QU San, ZHENG Cong, WANG Zai. A Research of The Stability Based on A Cell M odel Persistently Infected By A Field.isolated Virulent Strain of CSFV .VIROLOGICA SINICA, 2003, 18(3) : 271-274.

A Research of The Stability Based on A Cell M odel Persistently Infected By A Field.isolated Virulent Strain of CSFV

  • Available online: 18 June 2003
  • We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%.

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    A Research of The Stability Based on A Cell M odel Persistently Infected By A Field.isolated Virulent Strain of CSFV

    • 1. {1.College oflife sciences.Wuhan University,Wuhan 430072,China

    Abstract: We jointly applied immunofluorescence technique, RT-PCR assay and flow cytometer to evaluate the stability of a cell model persistently infected by Classical swine fever virus (CSFV39), which was established by our laboratory and named CSFV39-PK15. The experiment results indicated that this cell model was quite stable. Even in the 128th CSFV39-PK15 passage cells, we still could detect the existence of CSFV39. Both immunofluorescence antibody test and RT-PCR assay showed positive results. We also found there was no significant difference in their cell cycle between normal control PK-15 and CSFV39-PK15. Sequence homology analysis of the same fragment revealed that CSFV39 had the highest homology compared with Shimen strain, amounting to 99.02%.

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