Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus
Abstract: gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.