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Volume 18 Issue 3
June 2003
    Citation: DING Qiao, LI Jian-jun, CHEN Pu. Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus .VIROLOGICA SINICA, 2003, 18(3) : 275-278.
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    Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus

    • 1.

      Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095．China

    • 2.

      Department of Veterinary medicine,Foshan Science＆Technology College，Nanhai 528231，Chinaf1．Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095．China

    • Available online: 18 June 2003
    • gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.
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      Expression and Genic Imm unization of Glycoprotein gB Gene ofM arek’s disease virus

      • 1. Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095．China
      • 2. Department of Veterinary medicine,Foshan Science＆Technology College，Nanhai 528231，Chinaf1．Key Laboratory of Animal Diseases Diagnostic and Immunology of Agriculture Ministry of China,Nanjing Agricultural University,Nanjing 210095．China

      Abstract: gB gene of Marek’s disease virus (MDV) was inserted into pcDNA3, forming eukaryotic expression recombinant plasmid pcDNA3-gB. Recombinant plasmid pcDNA3-gB was identified by BamHⅠI and PCR. PcDNA3-gB was transfected into COS-7 cells in vitro. Expressed protein was detected by indirect immunofluorescence assay. Immune test groups were inoculated with pcDNA3-gB intramuscularly. Two weeks later, test groups were boosted with the same pcDNA3-gB. Since then, two weeks later chickens were challenged with vMDV. Immune effects showed that the immune protection rate of test group reached to 72.2%. The results proved that pcDNA3-gB DNA immunization elicited immune protection.

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