Citation: CHANG Guo—hui, PENG W en—ming, LIU Bo—hua, FAN Bao—chang, LIU Hong, DENG Yong—qiang, LU Fu—shuang, YANG Bao—an, QIN E-de, ZHU Qing—yu*. Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses .VIROLOGICA SINICA, 2003, 18(4) : 330-334.

Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses

  • Available online: 05 August 2003
  • Abstract:The viral RNAs were isolated from the infected Vero—E6 cells.then the templates were produced by reverse tran scription reaction.Two pairs of primers were used to amplify the 5 一ends of four strains of SAR. ssociated coronavirus(SARS—CoV)by RACE.cDNA fragments with the length of about 420 bp were amplified.The fragments were then purified an d sequenced.The sequences of 5 一UTRs of SARS —CoV isolated in China were the same as those isolated in other countries an d regions. such as Tor2 strain,Urbani strain,HKU—Su l 0 strain,CUHK—W l strain,S 2500 strain an d SIN2677 strain.Th e secondary structures formed by 5 一UTRs of all known SARS—CoV were almost identica1.but significan tly diferent from other kn own non—SARS coronaviruses.Sequence an alysis indicated that the conserved core sequence(5 一CUAAAC一3 1 of transcription regulating sequence in some coronaviruses was about—l 97 nt upstream from the start codon.

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    Comparative Analysis of Genomic Organization of the 5 and of Four Strain SARS—口ssociated Coronaviruses

    • 1. Institute of Microbiology and epidemiology,Academy ofMilitary Medical Sciences,Beijing 100071,China J

    Abstract: Abstract:The viral RNAs were isolated from the infected Vero—E6 cells.then the templates were produced by reverse tran scription reaction.Two pairs of primers were used to amplify the 5 一ends of four strains of SAR. ssociated coronavirus(SARS—CoV)by RACE.cDNA fragments with the length of about 420 bp were amplified.The fragments were then purified an d sequenced.The sequences of 5 一UTRs of SARS —CoV isolated in China were the same as those isolated in other countries an d regions. such as Tor2 strain,Urbani strain,HKU—Su l 0 strain,CUHK—W l strain,S 2500 strain an d SIN2677 strain.Th e secondary structures formed by 5 一UTRs of all known SARS—CoV were almost identica1.but significan tly diferent from other kn own non—SARS coronaviruses.Sequence an alysis indicated that the conserved core sequence(5 一CUAAAC一3 1 of transcription regulating sequence in some coronaviruses was about—l 97 nt upstream from the start codon.

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