Isolation and Identification of a Lethal Viral Pathogen From Com mon Cotton．eared M armosets

Abstract：As the close consan guinity of human race，the common cotton—eared marmoset(Callithrbc Jacchus)has been used in a wide range of research as a primate mode1．Acute respiratory infection(ARI) broke out in a colony reared in the experimental animal center of Tianjin Medical University in 1 999．A virus strain with high hemagglutinin titer was isolated out from embryonated eggs an d M DCK cells after inoculating with the lung tissues suspension of the dead marmosets． The data of the hemagglutination inhibition (HI)test with paried serum samples and the animal inoculation test confirmed that the isolated virus strain is the pathogen of the ARI．Th e isolated virus strain was then identified by the cross HI test。electron microscope observation。RT—PCR an d BLAST analyses．The virus was verified to be Sendai virus，which belongs to Parainfluenza virus type 1．virus was verified to be Sendai virus，which belongs to Parainfluenza virus type 1．