Citation: TAN Guo—lei, HU Zhi—hua, LU Ying—hua, CHEN De—sheng, CHEN Pu—yan. Construction of Eukarytic Expression Vector Containing PCV-2 ORF2 and Its Expression .VIROLOGICA SINICA, 2003, 18(4) : 367-370.

Construction of Eukarytic Expression Vector Containing PCV-2 ORF2 and Its Expression

  • Available online: 05 August 2003
  • According to the published OI 2 gene sequence of PCV一2.two primers were designed to amplify the ORF2 gene from PK一1 5 cells inoculated by PCV一2.It was cloned into the pSecTag2 vector,and the recombinant plasmid named pSecORF2 was obtained.Another primer was designed to amplify OI 2 gene including signal peptide sequence from pSecORF2.Then it was cloned into pIREShyg vector.Th e recombinan t expression vector plRESiORF2 was constructed.CHO cells were tran sfected in vitro with them. Th e expression of ORF2 gene was detected by indirect immunofluorescence assay(If1A、.Further research iS underway to study the biological activity of OI protein an d to establish the diagnostic kit of PCV

  • 加载中
  • 加载中

Article Metrics

Article views(3908) PDF downloads(986) Cited by()

Related
Proportional views

    Construction of Eukarytic Expression Vector Containing PCV-2 ORF2 and Its Expression

    • 1. College ofVeterinary Medicine,Nanjing Agriculture Universityt Nanfing 210095,China

    Abstract: According to the published OI 2 gene sequence of PCV一2.two primers were designed to amplify the ORF2 gene from PK一1 5 cells inoculated by PCV一2.It was cloned into the pSecTag2 vector,and the recombinant plasmid named pSecORF2 was obtained.Another primer was designed to amplify OI 2 gene including signal peptide sequence from pSecORF2.Then it was cloned into pIREShyg vector.Th e recombinan t expression vector plRESiORF2 was constructed.CHO cells were tran sfected in vitro with them. Th e expression of ORF2 gene was detected by indirect immunofluorescence assay(If1A、.Further research iS underway to study the biological activity of OI protein an d to establish the diagnostic kit of PCV

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return