Citation: XIE Yun—li, ZHANG Shu—yong, HUANG Ru, SHI Zheng—li*. A Modified Technique for Purifying White spot syndrome virus .VIROLOGICA SINICA, 2003, 18(4) : 391-393.

A Modified Technique for Purifying White spot syndrome virus

  • Available online: 05 August 2003
  • Abstract:The envelope proteins of White spot syndrome virus(WSSV)are very fragile and easy to be destroyed during purification.It was difi cult to obtain a large quan tity of intact virions by routine sucrose gradient centrifugation.After modifying the sucrose gradient by adding citrate sodium ,we can obtain a large quantity of intact virions and nucleocapsids.Th is purified virions and nucleocapsids were subsequently used for an alyzing viral structural proteins an d DNA extraction.Th e result showed that this modified technique is very efi cient for virus purification.

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    A Modified Technique for Purifying White spot syndrome virus

    • 1. Key laboratory of Molecular Virology,Joint·Laboratory of Invertebrate Hrology,Wuhan Institute of Hrology,Chinese Academy of Sciences,Wuhan 430071,China

    Abstract: Abstract:The envelope proteins of White spot syndrome virus(WSSV)are very fragile and easy to be destroyed during purification.It was difi cult to obtain a large quan tity of intact virions by routine sucrose gradient centrifugation.After modifying the sucrose gradient by adding citrate sodium ,we can obtain a large quantity of intact virions and nucleocapsids.Th is purified virions and nucleocapsids were subsequently used for an alyzing viral structural proteins an d DNA extraction.Th e result showed that this modified technique is very efi cient for virus purification.

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