Citation: HAO Yong—hua, KUANG Zhi—zhou, XU Yang. Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System .VIROLOGICA SINICA, 2003, 18(5) : 411-414.

Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System

  • Available online: 05 October 2003
  • A cDNA fragment of the vp8 gene from Rotavirus strain Wa was obtained by RT-PCR and cloned into the expressing vector pGEX-5x-1 to construct recombinant plasmid pGEX-VP8. Transformation of pGEX-VP8 to E.coli JM109 and positive clonse was select, The fragment vp8 was sequenced. With IPTG inducing, the recombimant productivity was detected by SDS-PAGE. The results demonstrated that the sequence of vp8 was correct and the productivity of VP8 recombinant protein was peaked after IPTG induction in 6-8h.

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    Expression of vp8 Gene of Rotavirus A in Prokaryotic Expression System

    • 1. Joint Research Institute of China—Germany in Jiangxi province,Key Laboratory of Food stuf of Chinese Education Ministry,Nachang 330047,China

    Abstract: A cDNA fragment of the vp8 gene from Rotavirus strain Wa was obtained by RT-PCR and cloned into the expressing vector pGEX-5x-1 to construct recombinant plasmid pGEX-VP8. Transformation of pGEX-VP8 to E.coli JM109 and positive clonse was select, The fragment vp8 was sequenced. With IPTG inducing, the recombimant productivity was detected by SDS-PAGE. The results demonstrated that the sequence of vp8 was correct and the productivity of VP8 recombinant protein was peaked after IPTG induction in 6-8h.

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