Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome
Abstract: Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS)and inf~ted by EB virus(EBV),in Order to establish immortalized B lymphoblastoid cell line(B—LCL). B—LCLs were then infected with the recombinant vaccinia viruse harboring Hantaan virus fHTNV)S gene.Expression of nucleocapsid protein was detected using indirect immunofluorescent assay(IFA). The results showed th at imm ortalized B—LCLs were set up.after B lymphocytes were infected by EBV an d cultured for 4 weeks.B lym phocytes tran sform ed by EBV becam e enlar ged an d further gath ered together.The HTNV nucleocapsid protein can be efectively expressed in B—LCLs仃ansinfected with recom—binant vaccinia viruse harboring HTNV-S gene.