Citation: WANG Ping—zhong, BAI Xue—Fan料, PAN Lei, ZHANG Ying, HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome .VIROLOGICA SINICA, 2003, 18(5) : 415-419.

Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

  • Available online: 05 October 2003
  • Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS)and inf~ted by EB virus(EBV),in Order to establish immortalized B lymphoblastoid cell line(B—LCL). B—LCLs were then infected with the recombinant vaccinia viruse harboring Hantaan virus fHTNV)S gene.Expression of nucleocapsid protein was detected using indirect immunofluorescent assay(IFA). The results showed th at imm ortalized B—LCLs were set up.after B lymphocytes were infected by EBV an d cultured for 4 weeks.B lym phocytes tran sform ed by EBV becam e enlar ged an d further gath ered together.The HTNV nucleocapsid protein can be efectively expressed in B—LCLs仃ansinfected with recom—binant vaccinia viruse harboring HTNV-S gene.

  • 加载中
  • 加载中

Article Metrics

Article views(4391) PDF downloads(818) Cited by()

Related
Proportional views

    Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

    • 1. The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi'an710038.China

    Abstract: Peripheral blood mononuclear cells(PBMCs)were separated on Ficoll—Hypaque density gradient centrifugation from patients with Hemorrhagic Fever with Renal Syndrome (HFRS)and inf~ted by EB virus(EBV),in Order to establish immortalized B lymphoblastoid cell line(B—LCL). B—LCLs were then infected with the recombinant vaccinia viruse harboring Hantaan virus fHTNV)S gene.Expression of nucleocapsid protein was detected using indirect immunofluorescent assay(IFA). The results showed th at imm ortalized B—LCLs were set up.after B lymphocytes were infected by EBV an d cultured for 4 weeks.B lym phocytes tran sform ed by EBV becam e enlar ged an d further gath ered together.The HTNV nucleocapsid protein can be efectively expressed in B—LCLs仃ansinfected with recom—binant vaccinia viruse harboring HTNV-S gene.

    Relative (20)

    目录

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return