Citation: WANG Ping—zhong, BAI Xue—Fan, PAN Lei, ZHANG Ying, HUANG Chang—xing. Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome .VIROLOGICA SINICA, 2003, 18(5) : 420-422.

Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

  • Available online: 05 October 2003
  • Abstract:In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG),gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector pCI.neo.The construction of pCI.neoGAG was confirmed by restriction endonuclease digestion(Xba I ISal I)analysis and DNA sequencing.Th en the pCI-neoGAG was transfected into p8 1 5 cells by lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection for 4 weeks.The results showed that the core protein was expressed in p8 1 5 cells successfully.which will be used for the further test of HIV.1 DNA vaccine.

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    Expression of Hantaan virus S Gene in B Lymphoblastoid Cell Lines from Patients with Hemorrhagic Fever with Renal Syndrome

    • 1. The Center of Diagnosis and Treatmentfor Infectious Diseases of Chinese PLA,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038.China

    Abstract: Abstract:In order to construct the eukaryotic expression vector of月“man immunodeficieacy virus 1 (HIV-I)core protein gene(pCI—neoGAG),gag gene was acquired from the plasmid pKSGAG by restriction endonuclease digestion (Xba I ISal I)and ligated onto the eukaryotic expression vector pCI.neo.The construction of pCI.neoGAG was confirmed by restriction endonuclease digestion(Xba I ISal I)analysis and DNA sequencing.Th en the pCI-neoGAG was transfected into p8 1 5 cells by lipofectine and the expressed product was detected by indirect immunofluorescence after G4 1 8 selection for 4 weeks.The results showed that the core protein was expressed in p8 1 5 cells successfully.which will be used for the further test of HIV.1 DNA vaccine.

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