Citation: TANG Shao, TANG Li, WANG Bin, Nobumichi Kobayashi, YANG Ji. Structural Protein rp 7 Gene Sequence Analysis of Human Group B Rotavirus Strain W H-2 .VIROLOGICA SINICA, 2003, 18(5) : 432-436.

Structural Protein rp 7 Gene Sequence Analysis of Human Group B Rotavirus Strain W H-2

  • Available online: 05 October 2003
  • Abstract:The VP7 gene(vp7)ofHuman group B rotavirus(GBRV)strain WH一2 was amplified and cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR).Positive vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee,the nucleotide sequence of WH一2 vp7 gene showed high identities(more than 9O%)to that of other human GBRV,with low identities(1ess than 65%)to that of animal GBRV.The secondary structure of WH-2 vp7 mRNA was constructed by software RNAstructure 3.7 an d RnaViz 2.0,18 stem—loop structures were found on it. Th e polypeptide encoded by W H一2 vp7 gene contains 249 amino acids with a calculated molecular weight of 28.4 kDa.The protein contains three potential N—linked glycosylation sites an d a hydrophobic signal—liked sequence at its amino termina1.Comparied with other GBRV,WH一2 vp7 demonstrated 99% an d 95% of am ino acids similarity with ADRV and CAL.1 respectively,only 5 1% witIl IDⅡ .Th ese findings suggested that W H一2 and ADRV might originate from a common an cestral virus,district from an imal W H一2 vp7 reported SO far.

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    Structural Protein rp 7 Gene Sequence Analysis of Human Group B Rotavirus Strain W H-2

    • 1. 1.Wuhan Centers for diseases Prevention and Control,Wuhan 430022,China

    Abstract: Abstract:The VP7 gene(vp7)ofHuman group B rotavirus(GBRV)strain WH一2 was amplified and cloned into the plasmid pUCm—T by reverse transcription polymerase chain reaction(RT-PCR).Positive vp7 gene cloned plasmid pT_VP7 was sequenced and analyzed by software GeneBee,the nucleotide sequence of WH一2 vp7 gene showed high identities(more than 9O%)to that of other human GBRV,with low identities(1ess than 65%)to that of animal GBRV.The secondary structure of WH-2 vp7 mRNA was constructed by software RNAstructure 3.7 an d RnaViz 2.0,18 stem—loop structures were found on it. Th e polypeptide encoded by W H一2 vp7 gene contains 249 amino acids with a calculated molecular weight of 28.4 kDa.The protein contains three potential N—linked glycosylation sites an d a hydrophobic signal—liked sequence at its amino termina1.Comparied with other GBRV,WH一2 vp7 demonstrated 99% an d 95% of am ino acids similarity with ADRV and CAL.1 respectively,only 5 1% witIl IDⅡ .Th ese findings suggested that W H一2 and ADRV might originate from a common an cestral virus,district from an imal W H一2 vp7 reported SO far.

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