Expression of HaSNPV sod gene in E．coli

Abstract：The coding region of superoxide dismutase was amplified by using PCR method from Helicoverpa armigera single enveloped nucleocapsid nucleopolyhedrovirus(HaSNPV)C 1 genome．The PCR product was cloned into pGEM —T-easy vector and sequenced．Th e cloned coding region of HaSNPV SOD was inserted into the expression vector pET blue一2 to form the recombinant plasmid pETblue2／HaSNPV SOD and was then transformed into E．coli DE3 for expression．The SDS—PAGE an alysis revealed that the expressed recombinan t SOD accumulated up to 37％ of the total bacterial protein．The enzyme activity of HaSNPV SOD was assayed by Pyrogallol autooxidation method．The result showed that the revised enzyme activity of the expressed product in the total soluble protein was 694 U／mg．