Citation: MA Zhong—liang, YANG Huai—yi, TIAN Bo*. Expression of Non—structural Protein S6 of Rice dwarf virus in E coil and Preparation of Its Antiserum .VIROLOGICA SINICA, 2003, 18(5) : 496-499.

Expression of Non—structural Protein S6 of Rice dwarf virus in E coil and Preparation of Its Antiserum

  • Available online: 05 October 2003
  • Abstract: gene of a Chinese isolate of Rice dwarf virus fRDV)was amplified by RT.PCR and then cloned into pGEM—Teasy.Sequence analysis showed that its nucleotide sequence had high identity to a Japan ese isolate of RDV an d contmned high percent of rare codons. l6 gene was expressed m inclusion body with high yield in E.coli after being subcloned into expression vector pGEX一6P—1.Immune rabbit witll S6 protein obtained the antiserum.The an tiserum having a titer of l:3000 tested by ELISA Can specifically react wim the expressed S6 protein.Our study confn-med that the an tiserum could be used to detect S6 protein in RDV-infected rice tissues.

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    Expression of Non—structural Protein S6 of Rice dwarf virus in E coil and Preparation of Its Antiserum

    • 1. Department of Molecular Virologogy,Institue ofMicrobiology,Chinese Academy of Sciences,Beijing 100080,China

    Abstract: Abstract: gene of a Chinese isolate of Rice dwarf virus fRDV)was amplified by RT.PCR and then cloned into pGEM—Teasy.Sequence analysis showed that its nucleotide sequence had high identity to a Japan ese isolate of RDV an d contmned high percent of rare codons. l6 gene was expressed m inclusion body with high yield in E.coli after being subcloned into expression vector pGEX一6P—1.Immune rabbit witll S6 protein obtained the antiserum.The an tiserum having a titer of l:3000 tested by ELISA Can specifically react wim the expressed S6 protein.Our study confn-med that the an tiserum could be used to detect S6 protein in RDV-infected rice tissues.

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