Efect of Human Cytomegalovirus on Proliferation of M ulfipotential Hematopoietic Progenitors in vitro

Abstract：To investigate the suppression effect of Human cytomegalovirus(HCMV)on multipotential hematopoietic progenitors(CFU—Mix)in vitro，colony forming unit—assay was applied to observe the effect of HCM V—AD169 strain on CFU—M ix of cord blood．1[11e technique of PCR Was used to demonstrate the existence of HCM V-DNA in the colony cells of cultured CFU-M ix．The numbers of CFU—M ix colonies in the groups infected with HCM V were significan tly less than that in control group， an d it showed an increase tendency with the decrease of HCMV concentration．1[11e suppression efect showed a dose—dependent fashion：the higher the HCM V．At)169 conce ntration．the more the CFU—Mix colonies decreased ．111e pcak of CFU—M ix colonies in control groups an d groups infected with HCM V appeared on the same time(10~12th day)，the lasting time of the CFU—Mix colonies in groups infected with HCM V Was significan tly shorter than that in control groups．HCNⅣ 一DNA Was positively detected in the colony cells of viral infected groups by PCR．while negative in the control groups．HC Ⅳ 一AD169 strain inhibited the diferentiation and proliferation of CFU—Mix by infec ting the hematopo ietic progenitors．HCMV may cause the suppression of hematopoiesis by direct infection，which may be the main reason of HCMV infection associated with anemia，neutropenia an d thrombocytope nia．