Citation: XU Li, SU Xiao, WANG Ling, SU Xin, REN Xue, SU Chun, CHEN Pu. Coexpression and Application ofN Gene ofPorcine reproductive and respiratory syndrome virus and HA gene ofInfluenza Virus .VIROLOGICA SINICA, 2003, 18(6) : 548-552.

Coexpression and Application ofN Gene ofPorcine reproductive and respiratory syndrome virus and HA gene ofInfluenza Virus

  • Available online: 25 December 2003
  • Abstract:From to the nucleotide sequences of haemoagglutinin gene of Influenza virus and Porcine R rD c and Respiratory Syndrome Virus(PRRSV)VR2332 in GenBank,a pair of primers which includes the main sequences of haemoagglutinin(HA)gene(33bp)was designed to amplify N gene (ORF7)of PRRSV by RT—PCR.The amplified fragment and pET一32a plasmid were digested by BamHI and XhoI.A recombinant plasmid nam ed pETHN was constructed and transformed into BL21(DE3). Consequently,the target protein was expressed by IPTG induction,and the purified fusion protein was obtained by His—binding purification kit.As a result latex—agglutination test Was set up an d plenty of serum sam ples were tested by the method.Th e results showed the method had same sensitivity and specificity and had 93.8% accord rate compared with ELISA(IDEXX)diagnosis kit.

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    Coexpression and Application ofN Gene ofPorcine reproductive and respiratory syndrome virus and HA gene ofInfluenza Virus

    • 1. 1.Key Laboratory ofAnimal Disease Diagnosis and Immunology,Nanjing Agricultural University,Nanjing 210095, China
    • 2. Department of Anima l Science,Agricultural College of Ningxia University,Yinchuan 750105 China

    Abstract: Abstract:From to the nucleotide sequences of haemoagglutinin gene of Influenza virus and Porcine R rD c and Respiratory Syndrome Virus(PRRSV)VR2332 in GenBank,a pair of primers which includes the main sequences of haemoagglutinin(HA)gene(33bp)was designed to amplify N gene (ORF7)of PRRSV by RT—PCR.The amplified fragment and pET一32a plasmid were digested by BamHI and XhoI.A recombinant plasmid nam ed pETHN was constructed and transformed into BL21(DE3). Consequently,the target protein was expressed by IPTG induction,and the purified fusion protein was obtained by His—binding purification kit.As a result latex—agglutination test Was set up an d plenty of serum sam ples were tested by the method.Th e results showed the method had same sensitivity and specificity and had 93.8% accord rate compared with ELISA(IDEXX)diagnosis kit.

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